Simona Patange, Biophysics PhD student
Hosted by Biophysics Program
Title: Quantitative single-cell imaging and optogenetic control of the c-MYC oncogene reveals its real-time effects on stochastic gene expression and transcription factor dynamics.
Time: 4:00PM - 5:00PM
Date: Monday, February 03, 2020
Abstract: How do transcription factors control gene expression in real time? To answer this question we examine c-MYC, arguably the most important and least understood oncogene in cancer. Several studies have recently cast doubt on the prevailing view of MYC as a gene-specific transcription factor—an 'on/off' switch for gene expression—and instead envision MYC as a non-linear amplifier—a 'volume knob'—to elevate the existing gene expression program in a cell. Here I summarize dissertation research co-advised by Daniel Larson (NIH), Michelle Girvan (UMD), and David Levens (NIH) that sheds light on the in vivo mechanism of MYC's amplification behavior by examining its real-time effects on stochastic transcription in living cells. The project is based on engineered optogenetic control of MYC, RNA imaging with single-molecule FISH and the MS2 system, and Halo-Tag imaging of transcription factor dynamics.
Gavin Bascom, Bolt Threads
Hosted by Sergei Sukharev
Title: What do Genes Look Like? Building Mesoscale Models of Developmentally Regulated Gene Hubs.
Time: 4:00PM - 5:00PM
Date: Monday, February 10, 2020
Abstract: How do transcription factors control gene expression in real time? To answer this question we examine c-MYC, arguably the most important and least understood oncogene in cancer. Several studies have recently cast doubt on the prevailing view of MYC as a gene-specific transcription factor—an 'on/off' switch for gene expression—and instead envision MYC as a non-linear amplifier—a 'volume knob'—to elevate the existing gene expression program in a cell. Here I summarize dissertation research co-advised by Daniel Larson (NIH), Michelle Girvan (UMD), and David Levens (NIH) that sheds light on the in vivo mechanism of MYC's amplification behavior by examining its real-time effects on stochastic transcription in living cells. The project is based on engineered optogenetic control of MYC, RNA imaging with single-molecule FISH and the MS2 system, and Halo-Tag imaging of transcription factor dynamics.
Michihiro Nagao, The National Institute of Standards and Technology (NIST)
Hosted by Robert Briber
Title: Thermal fluctuations and elastic and viscous properties in lipid bilayers measured by means of neutron spectroscopy.
Time: 4:00PM - 5:00PM
Date: Monday, February 24, 2020
Abstract: How do transcription factors control gene expression in real time? To answer this question we examine c-MYC, arguably the most important and least understood oncogene in cancer. Several studies have recently cast doubt on the prevailing view of MYC as a gene-specific transcription factor—an 'on/off' switch for gene expression—and instead envision MYC as a non-linear amplifier—a 'volume knob'—to elevate the existing gene expression program in a cell. Here I summarize dissertation research co-advised by Daniel Larson (NIH), Michelle Girvan (UMD), and David Levens (NIH) that sheds light on the in vivo mechanism of MYC's amplification behavior by examining its real-time effects on stochastic transcription in living cells. The project is based on engineered optogenetic control of MYC, RNA imaging with single-molecule FISH and the MS2 system, and Halo-Tag imaging of transcription factor dynamics.
Teresa Head-Gordon, University of California - Berkeley
SEMINAR HAS BEEN CANCELED
David Fushman, University of Maryland College Park
SEMINAR HAS BEEN CANCELED
Carlos Castaneda, Syracuse University
SEMINAR HAS BEEN CANCELED
Ruth Nussinov, The National Institutes of Health (NIH)
SEMINAR HAS BEEN CANCELED
Bin Wu, Johns Hopkins Medical School
SEMINAR HAS BEEN CANCELED